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Part:BBa_K415039:Design

Designed by: Shawn Pan   Group: iGEM10_MIT   (2010-10-26)


pBR322 Derived Low Copy pMB1 Origin Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 454


Design Notes

The extra reverse sequencing site in between the ROP and origin should be fixed in the future. Because of the sequence homologies with pUC19 origin backbones such as pSB1C3, it is impossible to specifically target the unwanted site with PCR without switching backbones.



Source

PCR extract from pSB6A1

References